Millipore Chip

RIPA Buffer (10X) - Cell Signaling Technology
Store at –20°C RIPA Buffer (10X) n 15 ml Description: RIPA buffer is used to lyse cells and tissues. 1X RIPA Buffer: 20 mM Tris-HCl (pH 7.5) 150 mM NaCl, 1 mM Na W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F ... View This Document

ChIP-Seq Protocol - Roadmap Epigenomics
ChIP-Seq Protocol: Tissue samples. Chromatin Precipitation (ChIP) using ~60ug of sheared chromatin equivalent. (Millipore #16-156 & #16-266) Day 1 (work on ice and use filtered tips) Pre-clear chromatin. Mix protein bead slurry well. Use a wide bore tip to aid in pipetting. ... Return Document

The Short-chain Fatty Acid Pentanoate Suppresses Autoimmunity By Modulating The Metabolic-epigenetic Crosstalk In Lymphocytes
Short-chain fatty acids (SCFAs) have immunomodulatory effects, but the underlying mechanisms are still unclear. Here the authors show that a SCFA, pentanoate, suppresses autoimmune inflammation ... Read News

WHSC1 Promotes Oncogenesis Through Regulation Of NIMA-Related ...
WHSC1 Promotes Oncogenesis through Regulation of NIMA-Related Kinase-7 in Squamous Cell Carcinoma of the Head and Neck Vassiliki Saloura1, formed using ChIP Assay kit (Millipore, 17–295) according to the manufacturer's protocol (26–29). Briefly, WHSC1 and frag- ... Access Document

Lab On A Chip - Pubs.rsc.org
On-a-chip applications for biomedical diagnostics.1–4 The big-gest drawback in the use of PDMS for these applications is its (A10-Merck-Millipore system, Darmstadt, Germany) was dropped onto the modified PDMS surface and digital images ... Retrieve Doc

CorepressorProteinCDYLFunctionsasaMolecularBridge ...
Ences catalogue no. 612667, ChIP Ab Millipore catalogue no. 17-662), SUZ12 (Cell Signaling catalogue no. 3737S), EED (Milliporecatalogueno.05-1320,ChIPAbMilliporecatalogue ... View Doc

Pittcon Editors' Awards - Wikipedia
Pittcon Editors’ Awards honoured the best new products on show at the Pittsburgh Conference on Analytical Chemistry and Texas Instruments Inc. - DLP4500NIR and the DLP NIRscan™ chip-based NIR polychromator and evaluation module EMD Millipore - Samplicity filtration system; ... Read Article

Supplemental Information Antagonistic Roles For H3K36me3 And ...
IgG (Millipore, PP64). All ChIP data were quantified by quantitative real-time PCR (Q-PCR). Values represent the average and SEM of three independent biological replicates in all cases. FLC expression was determined relative to UBC. ... Document Retrieval

Supplementary Information Lysine Demethylase LSD1 Coordinates ...
Supplementary Information Lysine demethylase LSD1 coordinates glycolytic and mitochondrial metabolism in hepatocellular carcinoma cells Akihisa Sakamoto, Shinjiro Hino, Katsuya Nagaoka, Kotaro Anan, Ryuta Takase, HIF-1α (Millipore (WB 1:200, ChIP), BD Biosciences (WB 1:1000) and Bethyl ... Fetch This Document

PericentricHeterochromatinGeneratedbyHP1Protein Interaction ...
ChIP dilution buffer (50 mM Tris-HCl, pH 8.0, 167 mM NaCl, 1.1% Triton X-100, 0.11% sodium deoxycholate) was added, and the samples were sonicated with Bioruptor (Cosmo Bio)(condition, power high, 15 s on and 30 s off for 24 cycles). After centrifugation (13,000 rpm for 10 min), the supernatant (500 l) was collected and combined with an ... Access Content

Identification Of B-catenin Binding Regions In Colon Cancer ...
Identification of b-catenin binding regions in colon cancer cells using ChIP-Seq Daniel Bottomly1, Sydney L. Kyler2, Shannon K. McWeeney1,3,4,5 and Gregory S. Yochum2,6,7,* 1Oregon Clinical and ... Fetch Full Source

ORC Figures PNAS - The Struhl Laboratory
ORC6 (Millipore) detection. (C) ChIP analysis of ORC2 binding at the MCM4 replication origin in ChIP-qPCR primers for the validation of ORC2 binding sites . Table S3. Common fragile sites are often located in large ORC2 "poor" regions of the genome . ... Access Doc

MiR-135 Suppresses Glycolysis And Promotes Pancreatic Cancer Cell Adaptation To Metabolic Stress By Targeting Phosphofructokinase-1
Pancreatic ductal adenocarcinoma must adapt to a nutrient-poor microenvironment. Here, the authors show that miR-135 accumulates in response to glutamine deprivation and inhibits aerobic glycolysis ... Read News

Lab On A Chip - ResearchGate
Lab on a Chip PAPER Cite this: Lab Chip,2014,14,4406 Milli-Q system (>18.2 MΩ cm−1) purchased from Millipore, was used for the preparation of all solutions. A thermostatic ... Retrieve Here

SUPPLEMENTAR Y INFORMATION - Media.nature.com
GAL4 Millipore 06-262 ChIP GAL4 Santa Cruz Sc-510 WB GFAP Abcam ab4674 IF H2AK119ub1 Cell Signaling 8240 1 WB, ChIP, ChIP-seq H3 Abcam ab1791 ChIP H3K4me3 Abcam ab8580 GR86940-1 ChIP, ChIP-seq H3K27ac Abcam ab4729 GR63945-1 ChIP-seq H3K27me3 Millipore 07-449 JBC1873477 ChIP, ChIP-seq ... View Document

Farnham Lab REMC ChIP And Library Construction Protocol
Farnham Lab REMC ChIP and Library Construction Protocol Preparation of c ross-linked cells (Record cell/patient information, date of Millipore* 9649s 07-352 3. Assess ChIP enrichments by quantitative PCR (qPCR) before proceeding to preparation ... Fetch Here

Histone H3.3K27M Mobilizes Multiple Cancer/Testis (CT ...
The EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit (Millipore; #17-10086) following the manufacturer's instruc-tion. Briefly, cells were cross-linked with 1% formaldehyde and sonicated to obtain DNA fragments between 0.3 and 1.0 kb. ... Doc Retrieval

Rethink Western blotting! See why you’ll get better outcomes when you use optimized tools for your Western blotting applications. ... View Video

Methods For Chromatin Analysis—Precipitating Protein, RNA ...
EMD Millipore. Temecula, CA . Genomics & Pharmacogenomics 2015. Confidential and Property of EMD Millipore Corporation. Chromatin Biology has come a long way… 1st gen ChIP Kit ~1999. Fully disclosed recipes. And protocol. 4th gen ChIP Kit. Every major supplier offers one. ... Doc Viewer

Calling Cards For DNA-Binding Proteins In Mammalian Cells
IgG (Millipore, EZMagna ChIP A kit) were used in the IP with 50 ml of Dyna1 protein G beads overnight at 4 . The precipi-tated immunocomplex was treated with proteinase K for 2 hr at 65 , and the DNA was purified with the Millipore EZMagna columns. Resultant DNA was prepared according to Illumina ’s protocol and sequenced on an Illumina GAIIx. ... Return Doc

ChIP-Seq Protocol - Roadmap Epigenomics
ChIP-Seq Protocol: Cells. Sonication via Diagenode sonicator Purify the reverse-crosslinked ChIP DNA sample using phase lock tubes and EtOH precipitation. protein A/G agarose beads (Millipore #16-156 & #16-266) Day 1 (work on ice and use filtered tips) Pre-clear chromatin. Mix protein ... Read Full Source

PRC2 Epigenetically Silences Th1-Type Chemokines To Suppress ...
PRC2 Epigenetically Silences Th1-Type Chemokines to Suppress Effector T-Cell Trafficking in Colon Cancer (ChIP) was performed and H3K27me3 (6002, Abcam) and IgG control (Millipore). ChIP-enrichedchromatinwasusedforRT-PCRwithSYBRGreenMaster Mix, normalizing to input. Specific primers ... Document Retrieval

Alterations Of Histone Modifications Contribute To Pregnane X ...
And transferred to polyvinylidene fluoride membranes (Millipore Sigma, Billerica, MA). Membranes were incubated with a primary dilution with ChIP dilution buffer (1 mM EDTA, 50 mM Tris-HCl, pH 8.0).AmouseimmunoglobulinG(IgG)(MilliporeSigma)wasusedasa ... Read Here

IMMUNOPRECIPITATION (IP) PROTOCOL - Abcam.com
Www.abcam.com/technical IMMUNOPRECIPITATION (IP) PROTOCOL Immunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of ... Get Document